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Interestingly, surface-modeling analyses revealed that the distance between the nuclei and the midline in in the right-side visceral muscles of Msp300ΔKASH embryos at T1-T4 was significantly less in than in wild type (Fig. Here, we have demonstrated that the bilaterally symmetric arrangement of the nuclei in the visceral muscles of the AMG is required for the LR-asymmetric development of this organ (Fig. Left Panel: Wilkinson. Nexus, the public body which owns Metro, commissioned the four artists in collaboration with train manufacturer Stadler and with funding from Arts Council England, following an open competition. I also spoke to many different people to collect this information before drawing each person for the panels. Drawing within shapes using Alpha Lock.

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Measuring the migration path of the nuclei. We manually traced the path of each nucleus through the sequence of images using the EP curve tool in Maya. We propose that the bilaterally symmetrical positioning of these nuclei may be mechanically coupled with subsequent LR-asymmetric morphogenesis. Artwork celebrates South Asian suffragettes and hidden figures in history ( part of the Narivad series) and a sneak peak into the makings of a matchbox book for the North East English Coast. Gauth Tutor Solution. A video showing how a matchbox book was created. This midline was used retrospectively for data captured at T1 to T3. Difference between CMYK compared to RGB. We previously reported that zipper2 (zip2), a mutant of the gene encoding MyoII heavy chain, produced a symmetrical AMG phenotype reminiscent of the Msp300ΔKASH mutant phenotype at a frequency of 60%, whereas LR defects rarely occurred in wild-type or zip2 heterozygous embryos (Fig. Abbie Robinson is a climber and coach who began pursuing her sport at her local climbing wall in Sunderland aged 13. The outline of the visceral muscles (green) was merged with the position of the nuclei (red spheres) using image analysis software (Fig.

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Craig played an important role in anti-racism campaigns and recent community projects and events, including Curious Arts and the NewBridge Project. Click to see the original works with their full license. 12 Free tickets every month. The 3D space files were imported into Maya version 2018 (Autodesk). Check the full answer on App Gauthmath. 1E, F), even though cell position changed very little over the 20 min imaging period. She addresses themes of social justice both in life and in her songs, and in 2019 was named Chancellor of the University of Sunderland.

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I'll also be creating a post where you can also find out how to access the Tyne and Wear archives and how I found my information for my research there. He came to the city on a lecture tour of Great Britain and Ireland and spoke to packed halls and churches. The densely crowded nuclei in these regions actively rearranged their positions relative to neighboring nuclei; hereafter, we refer to this as collective nuclear behavior. At stage 13, the anterior-posterior axis of the embryo (identified by head and tail structures), was manually reoriented to the y-axis of the image. The theme for the exhibition at Love of the North was Hyem this February so I designed this piece. In Lophotrochozoa and Ecdysozoa, intrinsic cell chirality plays a key role in LR-asymmetric development. As expected, UAS-dlp overexpression markedly suppressed these LR defects when driven by hand (frequency of LR defects 12%), 65E04 (19%) or 24B (12%) (Fig. To measure defects in collective nuclear behavior, we calculated a collectivity index to represent the mean distances between each nucleus and its nearest posterior neighbor, normalized as a percentage of the maximal width of the midgut (Fig.

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I added the details like the bricks up the sides and the circular shapes for the panels. I wanted to show the vital hidden history of the North East and to show some landmarks that are sometimes overlooked. In the surface-modeling analyses, visceral muscles are outlined in green, representing the outer surface of lifeact-EGFP distribution driven by 65E04-Gal4, a visceral muscle-specific Gal4 driver (Fig. We then averaged the mean values from 10 embryos (the average number of nuclei in each embryo was 20. Learning how to make your own letters. Considering potential differences in the size of the AMG, we normalized nucleus-midline distances as a ratio (percentage) relative to the maximum width of the AMG and calculated the mean of the normalized distances in each embryo (width of the blue box in Fig. We conducted a genetic screen that identified a new allele, dlp3, as a mutation that affects the LR-asymmetric development of the AMG (Fig. From these measurements, we calculated the average migration distance and standard deviation. I often feel these groups are under represented and wanted to show the vibrancy of their stories. Preprocessing the surface models. Alongside three other amazing artists I have won a unique commission to create major works for the interior of the Tyne and Wear Metro's £362m new train fleet. We used the GAL4/UAS system to drive the visceral muscle-specific expression of UAS-RedStinger, which encodes a nuclear DsRed, and of UAS-lifeact-EGFP, which encodes a GFP with an actin-binding peptide (Haralalka et al., 2014). We used Canton-S as the wild-type control strain. It was important for me to add different cultural backgrounds and also LGBTQ+ history as a queer artist.

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Metro will be the first urban transit system in the world to feature permanent art inside trains when the new fleet enters service next year, thanks to a project led by operator Nexus and funded by Arts Council England through its National Lottery Project Grants programme. In the mouse embryo, the clockwise rotation of the nodal cilia breaks bilateral symmetry by inducing the leftward flow of the extra-embryonic fluid (Shinohara et al., 2012). Analysis of LR-asymmetry in the AMG. Changing the background colour and removing backgrounds. Thus, MyoII and the LINC complex play important roles in the LR-symmetric rearrangement of the nuclei, which is required for or coupled with the subsequent LR-asymmetric morphogenesis (Fig. However, the average collectivity index did not differ significantly between zip2 and wild-type embryos, except for a slight reduction in the zip2 mutants at T1 (Fig. Frederick Douglass was an escaped slave from the United States who helped make Newcastle a leading part of the international abolition movement. We used this procedure to analyze the distance between the nuclei and the midline in wild-type and dlp3 homozygous embryos. Adele Emily Sandé MBE, known professionally as Emeli Sandé, is a singer and songwriter born in Sunderland to an English mother and Zambian father. The location of the nucleus, which is the largest organelle in the cell, changes as needed for various cellular contexts and functions (Gundersen and Worman, 2013). Deselecting and selecting layers. I really appreciate these moments where I can make fun bits of artwork of the North East fused with my own Punjabi heritage too! Cranes (now dismantled) from Swan Hunter's shipyard in Wallsend. Taken together, these results suggest an indispensable role for MyoII in preventing asymmetric nuclear positioning through stage 13, rather than in driving the subsequent LR-asymmetric morphogenesis that begins in stage 14.

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We also found that Wnt4 signaling is required for collective nuclear behavior in the visceral muscles (Fig. Indeed, the ubiquitous misexpression of UAS-dlp driven by arm-Gal4 in wild-type Drosophila causes LR defects, whereas control embryos carrying UAS-dlp but no Gal4 driver had no LR defects (Fig. It took a while and a couple of walks, and lush support/check from some Baltic staff who are familiar with the walk too to get it just right 🙂 You can colour it in and add your own doodles. When examining 2D snapshots projected from the 3D time-lapse movies, we noticed that the nuclei were more dispersed in dlp3 mutants than in wild-type embryos (Fig. To ensure no gaps on the piece I also added some textures to fill space. It became more about celebrating the diversity of culture, championing the strength of community and showcasing local history to inspire viewers.

The 'vampire rabbit' from Newcastle's Cathedral Buldings. The time-lapse images were saved as LSM files in ZEN software (2012 SP1 black edition, Release Version 8. How to pick a colour. We used Cy3-conjugated anti-mouse IgG (Jackson ImmunoResearch, 715-165-151; 1:500) and biotinylated anti-mouse IgG (Vector Labs, BA2000; 1:200) as secondary antibodies. Embryos were fixed and stained with anti-Fas3 (DSHB, 1:50) as described previously (Kuroda et al., 2012). To reduce phototoxicity, we used a relatively low laser power (488 nm laser, 0. The length of each traced path (μm) was then automatically measured using a Python script in Maya (see supplementary information). High-rise apartment blocks from Elswick in Newcastle. She went on to be a junior minister during the wartime coalition, and was Minister for Education from 1945 until her death in 1947.

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